|
| Section2 = }} Xylene cyanol can be used as a color marker, or tracking dye, to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose. Once mixed with the sample, concentration of xylene cyanol is typically about 0.005% to 0.03%. ==Migration speed== In 1% agarose gels, xylene cyanol migrates at about the same rate as a 4 to 5 kilobase pair DNA fragment 〔Lela Buckingham & Maribeth L. Flaws. ''Molecular Diagnostics.- Fundamentals, Methods, & Clinical Applications''. F.A. Davis Company, 2007, p. 91〕 (although this depends on the buffer used). Xylene cyanol on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment. On 20% denaturating (7 M urea) polyacrylamide gel electrophoresis (PAGE), xylene cyanol migrates at about the rate of 25 bases oligonucleotide. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Xylene cyanol」の詳細全文を読む スポンサード リンク
|